Year: 2018 | Month: February | Volume 11 | Issue 1
Genetic Diversity in Quercus leucotrichophora Populations Through RAPD Markers
Abstract:
Quercus leucotrichophora is an evergreen tree which is usually twisted and has wiry branches. Genetic diversity is an essential component for the effective implementation of a tree improvement program. Four populations collected from Jhungi Suket, Taradevi Shimla, Bhatwari-Taknor Range, Nohra-Shimla) of Q. leucotrichophora were studied using RAPD markers. Ten primers were screened out of which five showed polymorphism. Five primers were used to amplify the individuals from each population. In a multi population descriptive analysis, the total diversity (Ht) in the four populations of Quercus leucotrichophora ranged from 0.1620 to 0.3215 with the mean 0.2253. The within population gene diversity (Hs) ranged from 0.0928 to 0.2204 with the mean 0.1542. The among population differentiation (Gst) ranged from 0.0304 to 0.3177 with the mean 0.3155. The gene flow observed among the populations was 7.9677, which is greater than 1 indicating higher levels of gene flow in the studied populations. Highest similarity co-efficient of 0.1793 was observed between populations Jhungi Suket and Nohra Shimla. The highest percentage of polymorphic loci (89.06%) was found in the population from Jhungi Suket. RAPD markers indicated that the frequencies of the presence of these markers correlated with the result of UPGMA analysis. The genetic diversity of all the four populations from different regions were found to be low, so if the trees of one place is damaged by any means the trees of similar genetic diversity from other places could be planted. Genetic diversity also helps us to study the general characteristics of a species and also particular species can be free from diseases. In spite of the relatively short distances between populations three clearly distinct regional groups of populations could be identified. These findings have implications for decisions on in-situ and ex-situ genetic conservation and for forest management planning and practices.
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Print This Article Email This Article to Your Friend
